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KMID : 0377619650080050545
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Korean Jungang Medical Journal
1965 Volume.8 No. 5 p.545 ~ p.550
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Effect of acid and basis dyes on the activity of dysentery phage
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Abstract
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The papers reported about the action of dye to bacteriophage are rather few even though the data on bacteria have fairly been found.
The author has studied the effect of dye on the dysentery phage and the following results are obtained.
Many acid and basic dyes inactivated the activity of phage particle itself and the action of basic dyes, in general, seemed to be more strong than that of acid dyes.
The phage inactivating activities of dyes were most strong at 37℃ and, at 20℃ and 4℃, their actions were very weak or negligible. And also, the phage inactivating activities of dyes were not influenced by pH.
The phage adsorption to host cell was not influenced by dye (Crystal violet) and the appearance of phage adsorption occurred under the existence of dye was similar to that carried out in normal conditions.
The phage particles already adsorbed to host cell were not inactivated by the action of dye and a antagonistic phenomenon between the phage inactivating activity of acid and basic dye was found.
The mechanism of the action of dye was discussed.
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